A total of 1,195 players and club staff were tested in the sixth screening session, which took place on Thursday and Friday, and that yielded the second all-clear.
“The Premier League can confirm that on Thursday 4 June and Friday 5 June, 1,195 players and club staff were tested for Covid-19. Of these, zero have tested positive,” the league said in a statement.
The news is another step in the right direction for the league, which is scheduled to resume on 17 June.
There were six positive cases from three clubs in round one, two from two clubs in round two, a further four from two clubs in round three, none from round four and one from round five.
That means there have been a total of 13 positives from 6,274 tests administered.
Testing will continue on a twice-weekly basis.
When the action does resume, Premier League stadiums will be divided into three zones as clubs work towards agreeing matchday protocols.
Clubs met on Thursday to discuss some of the logistics and West Ham vice-chairman Karren Brady has revealed how stadiums will be split into three sections.
“We are now in the process of agreeing the matchday protocols, which will include everything from zoning the stadium into red, amber and green zones,” Brady said in her column in the Sun. “Red zone will be the most severely restricted area, including the pitch, the tunnel, technical areas, changing rooms will be limited to 105 people maximum, to include players, coaching staff, match officials and all the essential staff only. And only those who have tested negative for Covid-19 in the past five days can enter this area.
“We will be creating a Clinical Passport System for those who will need to have access to the red zone. The amber zone will be restricted to the minimum number of staff required to meet contractual requirements for broadcasting, media and club staff. This area includes all interior areas within the stadium and anyone entering this area will be subject to a temperature check and a health questionnaire. And the green zone is the stadium exterior, eg car parking.”